Positions

Selected Publications

Academic Article

Year Title Altmetric
2022 Validation of single-gene noninvasive prenatal testing for sickle cell diseaseAmerican Journal of Hematology.  97:E270-E273. 2022
2021 RNF2 ablation reprograms the tumor-immune microenvironment and stimulates durable NK and CD4+ T-cell-dependent antitumor immunity 2021
2021 A “Shot in the Arm” for Sickle Cell DiseaseMolecular Therapy.  29:416-417. 2021
2019 Hemoglobin β93 Cysteine Is Not Required for Export of Nitric Oxide Bioactivity from the Red Blood CellCirculation.  139:2654-2663. 2019
2019 A novel therapeutic strategy for b-thalassemiaBlood.  133:2245-2246. 2019
2019 Exogenous sickle erythrocytes combined with vascular disruption trigger disseminated tumor vaso-occlusion and lung tumor regressionJCI insight.  4. 2019
2018 Human leukocyte antigen class I and II knockout human induced pluripotent stem cell–derived cells: Universal donor for cell therapyJournal of the American Heart Association.  7. 2018
2018 Defining the epigenetic status of blood cells using a cyanine-based fluorescent probe for PRMT1Blood Advances.  2:2829-2836. 2018
2018 Bone Marrow Transplantation after Nonmyeloablative Treosulfan Conditioning Is Curative in a Murine Model of Sickle Cell DiseaseBiology of Blood and Marrow Transplantation.  24:1554-1562. 2018
2017 Elevated p53 Activities Restrict Differentiation Potential of MicroRNA-Deficient Pluripotent Stem CellsStem Cell Reports.  9:1604-1617. 2017
2017 Sickle cells produce functional immune modulators and cytotoxicsAmerican Journal of Hematology.  92:981-988. 2017
2017 Long-term endothelin-a receptor antagonism provides robust renal protection in humanized sickle cell disease miceJournal of the American Society of Nephrology.  28:2443-2458. 2017
2017 Induced pluripotent stem cell-derived endothelial cells overexpressing interleukin-8 receptors a/b and/or c-c chemokine receptors 2/5 inhibit vascular injury responseStem Cells Translational Medicine.  6:1168-1177. 2017
2017 Leukocyte iNOS is required for inflammation and pathological remodeling in ischemic heart failureBasic Research in Cardiology.  112. 2017
2016 Reprogramming by De-bookmarking the Somatic Transcriptional Program through Targeting of BET BromodomainsCell Reports.  16:3138-3145. 2016
2016 Novel HDAd/EBV Reprogramming Vector and Highly Efficient Ad/CRISPR-Cas Sickle Cell Disease Gene CorrectionScientific Reports.  6. 2016
2016 Sickle cells abolish melanoma tumorigenesis in hemoglobin SS knockin mice and augment the tumoricidal effect of oncolytic virus in vivoFrontiers in Oncology.  6. 2016
2016 The AS-RBM15 lncRNA enhances RBM15 protein translation during megakaryocyte differentiationEMBO Reports.  17:887-900. 2016
2016 The universal 3D3 antibody of human PODXL is pluripotent cytotoxic, and identifies a residual population after extended differentiation of pluripotent stem cellsStem Cells and Development.  25:556-568. 2016
2016 The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosisNature Communications.  7. 2016
2016 The histone H2A deubiquitinase Usp16 regulates hematopoiesis and hematopoietic stem cell functionProceedings of the National Academy of Sciences of USA.  113:E51-E60. 2016
2016 Endothelin-1 contributes to the progression of renal injury in sickle cell disease via reactive oxygen speciesBritish Journal of Pharmacology.  173:386-395. 2016
2015 Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicingeLife.  4. 2015
2015 Modeling Human Severe Combined Immunodeficiency and Correction by CRISPR/Cas9-Enhanced Gene TargetingCell Reports.  12:1668-1677. 2015
2015 Peroxiredoxin-2 recycling is inhibited during erythrocyte storageAntioxidants and Redox Signaling.  22:294-307. 2015
2014 The DPY30 subunit in SET1/MLL complexes regulates the proliferation and differentiation of hematopoietic progenitor cellsBlood.  124:2025-2033. 2014
2014 Broad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cellsPLoS One.  9. 2014
2014 The histone H2A deubiquitinase Usp16 regulates embryonic stem cell gene expression and lineage commitmentNature Communications.  5. 2014
2013 USP49 deubiquitinates histone H2B and regulates cotranscriptional pre-mRNA splicingGenes and Development.  27:1581-1595. 2013
2013 Hemopexin therapy improves cardiovascular function by preventing heme-induced endothelial toxicity in mouse models of hemolytic diseasesCirculation.  127:1317-1329. 2013
2013 Role of the b93cys, ATP and adenosine in red cell dependent hypoxic vasorelaxationInternational Journal of Physiology, Pathophysiology and Pharmacology.  5:21-31. 2013
2013 Antioxidant functions for the hemoglobin β93 cysteine residue in erythrocytes and in the vascular compartment in vivoFree Radical Biology and Medicine.  55:119-129. 2013
2012 FOXO1 orchestrates the bone-suppressing function of gut-derived serotoninJournal of Clinical Investigation.  122:3490-3503. 2012
2012 Activating transcription factor 4 mediates hyperglycaemia-induced endothelial inflammation and retinal vascular leakage through activation of STAT3 in a mouse model of type 1 diabetesDiabetologia.  55:2533-2545. 2012
2012 Heme-regulated eIF2α kinase activated Atf4 signaling pathway in oxidative stress and erythropoiesisBlood.  119:5276-5284. 2012
2012 Background mutations in parental cells account for most of the genetic heterogeneity of induced pluripotent stem cellsCell Stem Cell.  10:570-582. 2012
2012 SRY (sex determining region Y)-box2 (Sox2)/poly ADP-ribose polymerase 1 (Parp1) complexes regulate pluripotencyProceedings of the National Academy of Sciences of USA.  109:3772-3777. 2012
2011 Posttranscriptional control of type I interferon genes by KSRP in the innate immune response against viral infectionMolecular and Cellular Biology.  31:3196-3207. 2011
2011 PRC2 complexes with JARID2, MTF2, and esPRC2p48 in ES cells to modulate ES cell pluripotency and somatic cell reprogramingStem Cells.  29:229-240. 2011
2011 Negative regulation of IRF7 activation by activating transcription factor 4 suggests a cross-regulation between the IFN responses and the cellular integrated stress responsesJournal of Immunology.  186:1001-1010. 2011
2010 KLF1 regulates BCL11A expression and γ- to β-globin gene switchingNature Genetics.  42:742-744. 2010
2009 Atf4 regulates obesity, glucose homeostasis, and energy expenditureDiabetes.  58:2565-2573. 2009
2009 Polycistronic lentiviral vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cellsStem Cells.  27:1042-1049. 2009
2008 Global reorganization of replication domains during embryonic stem cell differentiationPLoS Biology.  6:2220-2236. 2008
2008 SNO-hemoglobin is not essential for red blood cell-dependent hypoxic vasodilationNature Medicine.  14:773-777. 2008
2008 ATF4 is an oxidative stress-inducible, prodeath transcription factor in neurons in vitro and in vivoJournal of Experimental Medicine.  205:1227-1242. 2008
2008 Gene replacement therapy for sickle cell disease and other blood disorders.Hematology online (ASH).  193-196. 2008
2007 Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skinScience.  318:1920-1923. 2007
2006 Correction of sickle cell disease by homologous recombination in embryonic stem cellsBlood.  108:1183-1188. 2006
2006 Differential binding of erythroid krupple-like factor to embryonic/fetal globin gene promoters during developmentJournal of Biological Chemistry.  281:16052-16057. 2006
2006 Alterations in expression and chromatin configuration of the alpha hemoglobin-stabilizing protein gene in erythroid Krüppel-like factor-deficient miceMolecular and Cellular Biology.  26:4368-4377. 2006
2005 Induction of KLF4 in basal keratinocytes blocks the proliferation- differentiation switch and initiates squamous epithelial dysplasiaOncogene.  24:1491-1500. 2005
2004 Excellent in vivo bystander activity of fludarabine phosphate against human glioma xenografts that express the Escherichia coli purine nucleoside phosphorylase geneCancer Research.  64:6610-6615. 2004
2004 A recombinant human hemoglobin with anti-sickling properties greater than fetal hemoglobinJournal of Biological Chemistry.  279:27518-27524. 2004
2004 ATF4 is a substrate of RSK2 and an essential regulator of osteoblast biology: Implication for Coffin-Lowry syndromeCell.  117:387-398. 2004
2004 Homocysteine Increases the Expression of Vascular Endothelial Growth Factor by a Mechanism Involving Endoplasmic Reticulum Stress and Transcription Factor ATF4Journal of Biological Chemistry.  279:14844-14852. 2004
2004 Rapid tagging of endogenous mouse genes by recombineering and ES cell complementation of tetraploid blastocysts.Nucleic Acids Research.  32. 2004
2003 Correction of a mouse model of sickle cell disease: Lentiviral/ antisickling β-globin gene transduction of unmobilized, purified hematopoietic stem cellsBlood.  102:4312-4319. 2003
2003 Nitric oxide-dependent generation of reactive species in sickle cell disease: Actin tyrosine nitration induces defective cytoskeletal polymerizationJournal of Biological Chemistry.  278:4194-4204. 2003
2003 Distinguishable live erythroid and myeloid cells in β-globin ECFP x lysozyme EGFP miceBlood.  101:903-906. 2003
2003 Designer Gene Therapy Using an Escherichia coli Purine Nucleoside Phosphorylase/Prodrug SystemChemistry and Biology.  10:1173-1181. 2003
2002 Basic residues within the Kruppel zinc finger DNA binding domains are the critical nuclear localization determinants of EKLF/KLF-1Journal of Biological Chemistry.  277:16304-16312. 2002
2002 Targeted disruption of the activating transcription factor 4 gene results in severe fetal anemia in miceBlood.  99:736-745. 2002
2002 Correction of phenotype in a thalassemia mouse model using a nonmyeloablative marrow transplantation regimenBiology of Blood and Marrow Transplantation.  8:453-461. 2002
2001 Oxygen radical inhibition of nitric oxide-dependent vascular function in sickle cell diseaseProceedings of the National Academy of Sciences of USA.  98:15215-15220. 2001
2001 Novel Transactivation Domain in Erythroid Kruppel-like Factor (EKLF)Journal of Biological Chemistry.  276:8239-8243. 2001
2001 Mouse model of congenital polycythemia: Homologous replacement of murine gene by mutant human erythropoietin receptor geneProceedings of the National Academy of Sciences of USA.  98:986-991. 2001
2000 Generation of novel embryonic stem cell lines from knockout-transgenic sickle cell disease mice: a new tool for therapeutic interventionBlood.  96. 2000
2000 Multiple transacnvation domains of erythroid kruppel-like factor (eklf)Blood.  96. 2000
2000 Human β-globin gene promoter element regulates human β-globin gene developmental specificityNucleic Acids Research.  28:2736-2740. 2000
2000 Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensationProceedings of the National Academy of Sciences of USA.  97:377-382. 2000
2000 Lentiviral vector transduction of hematopoietic stem cells that mediate long-term reconstitution of lethally irradiated miceStem Cells.  18:352-359. 2000
1999 Trichostatin A up-regulates human papillomavirus type 11 upstream regulatory region-E6 promoter activity in undifferentiated primary human keratinocytesJournal of Virology.  73:5026-5033. 1999
1998 Knockout-transgenic mouse models of human hemoglobinopathiesExperimental Hematology.  26:807. 1998
1998 double-replacement gene targeting in mouse embryonic stem cells - creation of a mouse model for congenital polycythemiaExperimental Hematology.  26:765. 1998
1998 Stochastic, stage-specific mechanisms account for the variegation of a human globin transgeneNucleic Acids Research.  26:2849-2858. 1998
1997 Knockout-transgenic mouse model of sickle cell diseaseScience.  278:873-876. 1997
1997 Reactivation of silenced, virally transduced genes by inhibitors of histone deacetylaseProceedings of the National Academy of Sciences of USA.  94:5798-5803. 1997
1997 The bZIP transcription factor LCR-F1 is essential for mesoderm formation in mouse developmentGenes and Development.  11:786-798. 1997
1996 Activation of δ-globin gene expression by erythroid Krupple-like factor: A potential approach for gene therapy of sickle cell diseaseBlood.  88:4051-4057. 1996
1996 Stable transduction of recombinant adeno-associated virus into hematopoietic stem cells from normal and sickle cell patients.Biology of Blood and Marrow Transplantation.  2:24-30. 1996
1996 Stable transduction of recombinant adeno-associated virus into hematopoietic stem cells from normal and sickle cell patientsBiology of Blood and Marrow Transplantation.  2:24-30. 1996
1995 Mouse model of human β0 thalassemia: Targeted deletion of the mouse β(maj)- and β(min)-globin genes in embryonic stem cellsProceedings of the National Academy of Sciences of USA.  92:9259-9263. 1995
1995 Autonomous, erythroid-specific DNAse I hypersensitive site formed by human β-globin locus control region (LCR) 5' HS 2 in transgenic miceDevelopmental Biology.  169:728-732. 1995
1995 End joining of genomic DNA and transgene DNA in fertilized mouse eggsGene.  165:173-181. 1995
1995 Role of erythroid Kruppel-like factor in human γ- to β-globin gene switchingJournal of Biological Chemistry.  270:1955-1959. 1995
1994 Retroviral vector sequences inhibit human β-globin gene expression in transgenic miceNucleic Acids Research.  22:4477-4481. 1994
1994 Recombinant human hemoglobins designed for gene therapy of sickle cell diseaseProceedings of the National Academy of Sciences of USA.  91:9852-9856. 1994
1994 Cloning and functional characterization of LCR-F1: A bZIP transcription factor that activates erythroid-specific, human globin gene expressionNucleic Acids Research.  22:2383-2391. 1994
1994 Multiple elements in human β-globin locus control region 5′ HS 2 are involved in enhancer activity and position independent, transgene expressionNucleic Acids Research.  22:1006-1011. 1994
1994 Preparation of recombinant hemoglobin in transgenic miceMethods in Enzymology.  231:403-434. 1994
1991 Human β-globin locus control region: Analysis of the 5' DNase I hypersensitive site HS 2 in transgenic miceProceedings of the National Academy of Sciences of USA.  88:1626-1630. 1991
1991 Survival and integration rate of channel catfish and common carp embryos microinjected with DNA at various developmental stagesAquaculture.  99:249-255. 1991
1990 Human globin locus activation region (LAR): role in temporal controlTrends in Genetics.  6:219-222. 1990
1990 Human sickle hemoglobin in transgenic miceScience.  247:566-568. 1990
1990 Human γ- To β-globin gene switching in transgenic miceGenes and Development.  4:380-389. 1990
1989 A single erythroid-specific DNase I super-hypersensitive site activates high levels of human beta-globin gene expression in transgenic mice.Genes and Development.  3:314-323. 1989
1989 DNase I super-hypersensitive sites direct high level erythroid expression of human alpha-, beta- and beta s- globin genes in transgenic mice.Progress in clinical and biological research.  316 A:47-61. 1989
1989 High-level erythroid expression of human α-globin genes in transgenic miceProceedings of the National Academy of Sciences of USA.  86:37-41. 1989
1989 Synthesis of functional human hemoglobin in transgenic miceScience.  245:971-973. 1989
1987 Multiple sequences regulate human beta-globin gene expression in transgenic mice.Progress in clinical and biological research.  251:323-343. 1987
1987 Transfer of the metallothionein-human growth hormone fusion gene into channel catfishTransactions of the American Fisheries Society.  116:87-91. 1987
1987 Two 3' sequences direct adult erythroid-specific expression of human β-globulin genes in transgenic miceProceedings of the National Academy of Sciences of USA.  84:7056-7060. 1987
1985 Erythroid-specific expression of human beta-globin genes in transgenic mice.The EMBO Journal.  4:1715-1723. 1985
1985 Expression of human β-globin genes in transgenic mice: effects of a flanking metallothionein-human growth hormone fusion geneMolecular and Cellular Biology.  5:1977-1983. 1985
1985 Structural organization of the alpha and beta globin loci of the goat.Progress in clinical and biological research.  191:67-79. 1985
1984 Duplication of a new four-gene set during the evolution of the goat β-globin locus produced genes now expressed differentially in developmentJournal of Biological Chemistry.  259:1896-1900. 1984
1984 Triplication of a four-gene set during evolution of the goat β-globin locus produced three genes now expressed differentially during developmentProceedings of the National Academy of Sciences of USA.  81:6589-6593. 1984
1983 Organization, structure, and expression of the goat globin genes.Progress in clinical and biological research.  134:131-139. 1983
1983 Sequence and linkage of the goat εI and εII β-globin genesJournal of Molecular Biology.  169:31-52. 1983
1982 DNA content, kinetic complexity, and the ploidy question in Candida albicansMolecular and Cellular Biology.  2:853-862. 1982
1977 Protection by cycloheximide from hyperthermic inhibition of protein synthesis in human reticulocytesLife Sciences.  20:585-591. 1977
1977 Effect of dibutyryl cyclic adenosine 5' monophosphate on protein synthesis in L5178Y cells after hyperthermiaJournal of the National Cancer Institute.  59:1469-1473. 1977

Teaching Activities

  • 01-115 - CMB1 BIOMOLECULES (Fall Term 2007)
  • 01-115 - CMB1 BIOMOLECULES (Fall Term 2008)
  • 01-115 - CMB1 BIOMOLECULES (Fall Term 2009)
  • BMG726 - BMG Seminar (Spring Term 2012)
  • BMG726 - BMG Seminar (Spring Term 2013)
  • BMG726 - BMG Seminar (Spring Term 2014)
  • BMG769 - Graduate Student Research Mtg (Fall Term 2012)
  • BMG769 - Graduate Student Research Mtg (Spring Term 2012)
  • BMG769 - Graduate Student Research Mtg (Spring Term 2013)
  • BMG796 - Journal Club Adv Euk Mole Biol (Fall Term 2007)
  • BMG796 - Journal Club Adv Euk Mole Biol (Fall Term 2008)
  • BMG796 - Journal Club Adv Euk Mole Biol (Fall Term 2009)
  • BMG796 - Journal Club Adv Euk Mole Biol (Fall Term 2010)
  • BMG796 - Journal Club Adv Euk Mole Biol (Fall Term 2011)
  • BMG796 - Journal Club Adv Euk Mole Biol (Fall Term 2012)
  • BMG796 - Journal Club Adv Euk Mole Biol (Fall Term 2013)
  • BMG796 - Journal Club Adv Euk Mole Biol (Spring Term 2008)
  • BMG796 - Journal Club Adv Euk Mole Biol (Spring Term 2009)
  • BMG796 - Journal Club Adv Euk Mole Biol (Spring Term 2010)
  • BMG796 - Journal Club Adv Euk Mole Biol (Spring Term 2011)
  • BMG796 - Journal Club Adv Euk Mole Biol (Spring Term 2012)
  • BMG796 - Journal Club Adv Euk Mole Biol (Spring Term 2013)
  • BMG796 - Journal Club Adv Euk Mole Biol (Spring Term 2014)
  • BMG798 - Non-Dissertation Research (Fall Term 2008)
  • BMG798 - Non-Dissertation Research (Fall Term 2009)
  • BMG798 - Non-Dissertation Research (Fall Term 2010)
  • BMG798 - Non-Dissertation Research (Fall Term 2011)
  • BMG798 - Non-Dissertation Research (Fall Term 2012)
  • BMG798 - Non-Dissertation Research (Spring Term 2009)
  • BMG798 - Non-Dissertation Research (Spring Term 2010)
  • BMG798 - Non-Dissertation Research (Spring Term 2011)
  • BMG798 - Non-Dissertation Research (Spring Term 2012)
  • BMG798 - Non-Dissertation Research (Spring Term 2013)
  • BMG798 - Non-Dissertation Research (Summer Term 2008)
  • BMG798 - Non-Dissertation Research (Summer Term 2009)
  • BMG798 - Non-Dissertation Research (Summer Term 2010)
  • BMG798 - Non-Dissertation Research (Summer Term 2011)
  • BMG798 - Non-Dissertation Research (Summer Term 2012)
  • BMG798 - Non-Dissertation Research (Summer Term 2013)
  • BMG799 - Dissertation Research (Fall Term 2008)
  • BMG799 - Dissertation Research (Fall Term 2009)
  • BMG799 - Dissertation Research (Fall Term 2010)
  • BMG799 - Dissertation Research (Fall Term 2011)
  • BMG799 - Dissertation Research (Fall Term 2012)
  • BMG799 - Dissertation Research (Spring Term 2009)
  • BMG799 - Dissertation Research (Spring Term 2010)
  • BMG799 - Dissertation Research (Spring Term 2011)
  • BMG799 - Dissertation Research (Spring Term 2012)
  • BMG799 - Dissertation Research (Spring Term 2013)
  • BMG799 - Dissertation Research (Summer Term 2008)
  • BMG799 - Dissertation Research (Summer Term 2009)
  • BMG799 - Dissertation Research (Summer Term 2010)
  • BMG799 - Dissertation Research (Summer Term 2011)
  • BMG799 - Dissertation Research (Summer Term 2012)
  • BMG799 - Dissertation Research (Summer Term 2013)
  • CMB713 - CMB I Biomolecules (Fall Term 2007)
  • CMB713 - CMB I Biomolecules (Fall Term 2008)
  • CMB713 - CMB I Biomolecules (Fall Term 2009)
  • GBS780 - BSB Lab Methods (Fall Term 2010)
  • GBS780 - BSB Lab Methods (Fall Term 2011)
  • GBS780 - BSB Lab Methods (Fall Term 2012)
  • GBS788 - Special Topics (Fall Term 2015)
  • GBSC700 - Journal Clubs (Fall Term 2014)
  • GBSC700 - Journal Clubs (Fall Term 2015)
  • GBSC700 - Journal Clubs (Fall Term 2016)
  • GBSC700 - Journal Clubs (Fall Term 2017)
  • GBSC700 - Journal Clubs (Fall Term 2018)
  • GBSC700 - Journal Clubs (Spring Term 2015)
  • GBSC700 - Journal Clubs (Spring Term 2016)
  • GBSC700 - Journal Clubs (Spring Term 2017)
  • GBSC700 - Journal Clubs (Spring Term 2018)
  • GBSC700 - Journal Clubs (Spring Term 2019)
  • GBSC701 - Seminars (Spring Term 2015)
  • GBSC709 - Adv Stem Cell/Regen Med (Fall Term 2014)
  • GBSC735 - Discoveries in Mol Bio (Fall Term 2018)
  • Education And Training

  • Master of Arts in Zoology / Animal Biology, University of Tennessee System : Knoxville 2000
  • Doctor of Philosophy in Zoology / Animal Biology, University of Tennessee System : Knoxville 1980
  • Bachelor's Degree in Microbiology, University of Tennessee System : Knoxville 1973
  • Full Name

  • Tim Townes