Purnima Singh

Purnima Singh
Associate Professor

Member (C), Institute for Cancer Outcomes & Survivorship , School of Medicine
Associate Scientist (C), Center for Clinical and Translational Science (CCTS) , General Clinical Research Center
Associate Professor (P), Pediatric - Hematology-Oncology , Pediatrics

Overview

Purnima Singh, MSc, PhD, MSPH joined UAB in 2015 as a member of The Institute for Cancer Outcomes and Survivorship. She is an Associate Professor in the Division of Pediatric - Hematology-Oncology. Following a PhD in Molecular Biology and Biotechnology from G. B. Pant University, India, Dr. Singh completed her postdoctoral training at City of Hope Comprehensive Cancer Center, CA in DNA Replication and Repair (Dr. Binghui Shen) and Developmental Epigenetics (Dr. Piroska Szabo). She was a Research faculty at the Dept. of Population Sciences at City of Hope. Her current goal is to understand the molecular and epigenetic basis of adverse outcomes in childhood cancer survivors by following an integrative genomics approach, to allow for targeted therapy, thus reducing morbidity in children with cancer.

Selected Publications

Academic Article

Year	Title	Altmetric
2023	Genome-Wide Association Study Identifies ROBO2 as a Novel Susceptibility Gene for Anthracycline-Related Cardiomyopathy in Childhood Cancer Survivors.. Journal of Clinical Oncology. 41:1758-1769. 2023
2023	Haptoglobin Gene Expression and Anthracycline-Related Cardiomyopathy in Childhood Cancer Survivors A COG-ALTE03N1 Report. JACC. CardioOncology. 2023
2022	Genome-wide variants and polygenic risk scores for cognitive impairment following blood or marrow transplantation. Bone Marrow Transplantation. 57:925-933. 2022
2022	Immunogenicity and safety of the human papillomavirus vaccine in young survivors of cancer in the USA: a single-arm, open-label, phase 2, non-inferiority trial. Lancet Child and Adolescent Health. 6:38-48. 2022
2021	EHMT2 suppresses the variation of transcriptional switches in the mouse embryo. PLoS Genetics. 17. 2021
2021	EHMT2 Controls Transcriptional Noise and the Developmental Switch after Gastrulation in the Mouse Embryo 2021
2021	Prenatal correction of IGF2 to rescue the growth phenotypes in mouse models of Beckwith-Wiedemann and Silver-Russell syndromes. Cell Reports. 34. 2021
2020	Association of GSTM1 null variant with anthracycline-related cardiomyopathy after childhood cancer—A Children's Oncology Group ALTE03N1 report. Cancer. 126:4051-4058. 2020
2020	GSTM1 null variant associated with anthracycline-related cancer in pediatric cancer.. Cancer. 126:3926-3928. 2020
2020	Genome Wide Association Study (GWAS) of cognitive impairment after blood or marrow transplantation (BMT) for hematologic malignancy.. Journal of Clinical Oncology. 38:1536-1536. 2020
2020	Clinical and genetic risk prediction of cognitive impairment after blood or marrow transplantation for hematologic malignancy. Journal of Clinical Oncology. 38:1312-1321. 2020
2020	Isolation of DNA-free RNA from human bone marrow mononuclear cells: Comparison of laboratory methods 2020
2019	A novel association between GSTM1 null variant and anthracycline-induced cardiac dysfunction (ACD) in childhood cancer survivors (CCS): A COG ALTE03N1 report.. Journal of Clinical Oncology. 37:10030-10030. 2019
2019	Candidate SNPs enhance prediction of cognitive impairment after blood or marrow transplantation (BMT) for hematologic malignancy (HM).. Journal of Clinical Oncology. 37:11520-11520. 2019
2019	Risk prediction of anthracycline-related cardiomyopathy (AC) in childhood cancer survivors (CCS): A COG-ALTE03N1 and CCSS report.. Journal of Clinical Oncology. 37:10015-10015. 2019
2018	Factors associated with physical inactivity in adult breast cancer survivors—A population-based study. Cancer Medicine. 7:6331-6339. 2018
2017	Clinical and genetic risk prediction of subsequent CNS tumors in survivors of childhood cancer: A report fromthe COG ALTE03N1 study. Journal of Clinical Oncology. 35:3688-3696. 2017
2016	Single Nucleotide Polymorphisms (SNPs) Associated with Cognitive Impairment in Patients Treated with Hematopoietic Cell Transplantation (HCT): A Longitudinal Study. Blood. 128:824-824. 2016
2016	High type I error and misrepresentations in search for transgenerational epigenetic inheritance: Response to Guerrero-Bosagna. Genome Biology. 17. 2016
2016	CELF4 variant and anthracycline-related cardiomyopathy: A children's oncology group genome-wide association study. Journal of Clinical Oncology. 34:863-870. 2016
2015	Draft genome of Cryobacterium sp. MLB-32, an obligate psychrophile from glacier cryoconite holes of high Arctic. Marine Genomics. 21:25-26. 2015
2015	CELF4 variant and Anthracycline-related Cardiomyopathy (anth-card) – A COG Study (ALTE03N1).. Journal of Clinical Oncology. 33:10066-10066. 2015
2015	Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming. Genome Biology. 16. 2015
2014	Characterization of the imprinting signature of mouse embryo fibroblasts by RNA deep sequencing. Nucleic Acids Research. 42:1772-1783. 2014
2013	De Novo DNA Methylation in the Male Germ Line Occurs by Default but Is Excluded at Sites of H3K4 Methylation. Cell Reports. 4:205-219. 2013
2012	More than insulator: Multiple roles of CTCF at the H19-Igf2 imprinted domain. Frontiers in Genetics. 3. 2012
2012	Chromatin immunoprecipitation to characterize the epigenetic profiles of imprinted domains. Methods in Molecular Biology. 925:159-172. 2012
2012	Chromatin immunoprecipitation to characterize the epigenetic profiles of imprinted domains.. Methods in Molecular Biology. 925:159-172. 2012
2011	Chromosome-wide analysis of parental allele-specific chromatin and DNA methylation. Molecular and Cellular Biology. 31:1757-1770. 2011
2011	Effects of endocrine disruptors on imprinted gene expression in the mouse embryo. Epigenetics. 6:937-950. 2011
2011	MIRA-SNuPE, a quantitative, multiplex method for measuring allele-specific DNA methylation. Epigenetics. 6:212-223. 2011
2010	Coordinated allele-specific histone acetylation at the differentially methylated regions of imprinted genes. Nucleic Acids Research. 38:7974-7990. 2010
2010	CTCF-dependent chromatin bias constitutes transient epigenetic memory of the mother at the H19-igf2 imprinting control region in prospermatogonia. PLoS Genetics. 6. 2010
2010	Complete biallelic insulation at the H19/Igf2 imprinting control region position results in fetal growth retardation and perinatal lethality. PLoS One. 5:1-11. 2010
2010	Allele-specific H3K79 di- versus trimethylation distinguishes opposite parental alleles at imprinted regions. Molecular and Cellular Biology. 30:2693-2707. 2010
2009	The 3′-flap pocket of human flap endonuclease 1 is critical for substrate binding and catalysis. Journal of Biological Chemistry. 284:22184-22194. 2009
2008	Overexpression and hypomethylation of Flap endonuclease 1 gene in breast and other cancers. Molecular Cancer Research. 6:1710-1717. 2008
2008	Comprehensive Mapping of the C-Terminus of Flap Endonuclease-1 Reveals Distinct Interaction Sites for Five Proteins That Represent Different DNA Replication and Repair Pathways. Journal of Molecular Biology. 377:679-690. 2008
2007	Fen1 mutations result in autoimmunity, chronic inflammation and cancers. Nature Medicine. 13:812-819. 2007
2007	Concerted action of exonuclease and gap-dependent endonuclease activities of FEN-1 contributes to the resolution of triplet repeat sequences (CTG) n- and (GAA)n-derived secondary structures formed during maturation of Okazaki fragments. Journal of Biological Chemistry. 282:3465-3477. 2007
2005	Characterization of the 3′ → 5′ exonuclease activity found in human nucleoside diphosphate kinase 1 (NDK1) and several of its homologues. Biochemistry. 44:15774-15786. 2005
2005	Multiple but dissectible functions of FEN-1 nucleases in nucleic acid processing, genome stability and diseases. BioEssays. 27:717-729. 2005
2005	Dual negative regulatory mechanisms of RecX on RecA functions in radiation resistance, DNA recombination and consequent genome instability in Deinococcus radiodurans. DNA Repair. 4:671-678. 2005
2003	Enhanced transformation of plant cells following co-bombardment of VirE2 protein of Agrobacterium tumefaciens with DNA substrate. Current Science. 85:1343-1347. 2003
2003	Transient expression of foreign genes in mature wheat embryo explants following particle bombardment. Physiology and Molecular Biology of Plants. 9:217-223. 2003

Chapter

Year	Title	Altmetric
2007	Nucleases in DNA repair, replication and recombination: Flap endonuclease-1 as a paradigm. 267-300. 2007

Research Overview

As an integral member of the Institute for Cancer Outcomes and Survivorship at UAB, my goal is to understand the molecular and epigenetic basis of adverse outcomes in childhood cancer survivors. Anthracycline-related cardiomyopathy and congestive heart failure is the leading cause of late morbidity in survivors of childhood cancers. There is a dose-dependent association between anthracycline exposure and cardiomyopathy risk, suggesting that genetic predisposition possibly modifies this association. I am following an integrative genomics approach to understand whether genetic and epigenetic predisposition can place childhood cancer survivors at an increased risk for developing treatment-related complications and therefore allow for targeted therapy, thus reducing morbidity in children with cancer.