R-spondin1 synergizes with Wnt3A in inducing osteoblast differentiation and osteoprotegerin expression

Academic Article


  • R-spondins are a new group of Wnt/β-catenin signaling agonists, however, the role of these proteins in bone remains unclear. We reported herein that R-sponin1 (Rspo1) acted synergistically with Wnt3A to activate Wnt/β-catenin signaling in the uncommitted mesenchymal C2C12 cells. Furthermore, we found that Rspo1 at concentrations as low as 10 ng/ml synergized strongly with Wnt3A to induce C2C12 osteoblastic differentiation and osteoprotegerin expression. These events were blocked by Wnt/β-catenin signaling antagonist Dickkopf-1. Finally, we demonstrated that Rspo1 synergized with Wnt3A to induce primary mouse osteoblast differentiation. Together, these findings suggest that Rpos1 may play an important role in bone remodeling. © 2008 Federation of European Biochemical Societies.
  • Authors

    Published In

  • FEBS Letters  Journal
  • Digital Object Identifier (doi)

    Author List

  • Lu W; Kim KA; Liu J; Abo A; Feng X; Cao X; Li Y
  • Start Page

  • 643
  • End Page

  • 650
  • Volume

  • 582
  • Issue

  • 5