Ontogeny of Ia and IgD on IgM-bearing B lymphocytes in mice*

Academic Article


  • We used immunofluorescence to examine the developmental relationship of Ia and IgD on B cells. Pre-B cells in fetal liver did not express la. Only very few surface IgM-positive (sIgM+) B cells in fetal spleen were found to be Ia+ and were weakly stained for la. After birth there was a linear increase in the proportion of sIgM+ spleen cells which expressed la, reaching -95% by 9 days. Adult bone marrow also contains a sizeable proportion of sIgM+ Ia+ cells. Unstimulated cells from fetal or newborn liver and spleen expressed Ia at the same rate in culture. Anti-la antisera suppressed the LPS-induced differentiation of IgM and IgG plasma cells in cultures of neonatal lymphocytes. Ia was also detected on IgM and IgG plasma cells in vitro suggesting that lipopolysac-charide (LPS)-stimulated B cells may express Ia antigens, induced by LPS, or appearing as part of normal differentiation. IgD did not appear on sIgM+ cells until 3 days of age and then rose slowly to reach adult levels later than Ia antigens. © American Society for Clinical Pathology.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Kearney JF; Cooper MD; Klein J; Abney ER; Parkhouse RME; Lawton AR
  • Start Page

  • 297
  • End Page

  • 301
  • Volume

  • 146
  • Issue

  • 1