Enhancement of in Vitro Immune Responses of Murine Peyer's Patch Cultures by Concanavalin A, Muramyl Dipeptide and Lipopolysaccharide

Academic Article


  • Immunofluorcscent and esterase staining of Peyer's patch (PP) cell preparations from six mouse strains showed that each strain possessed approximately eqtial numbers of T and B cells but less than 1% esterase‐positive cells (macrophages; Mψ). The addition of concanavalin A (Con A) to either lipopolysaccharide (LPS)‐responsive C3H/HeN or LPS‐non‐responsive C3H/Hej PP cultures immunized with sheep erythrocytes (SRBC) resulted in good anti‐SRBC plaque‐forming cell responses, whereas the addition of Con A to PP cultures from SRBC orally primed C3H/HeJ mice resulted in significantly higher in vitro responses to trinitrophenyl‐conjugated SRBC than similarly treated cultures from C3H/HeN mice. On the other hand, muramyl dipeptide (MDP) promoted higher responses in PP cultures derived from either normal or carrier‐primed LPS‐rcsponsive (C3H/HeN) mice than were observed with C3H/HeJ PP cultures. A similar pattern of responsiveness was seen when LPS prepared by a phenol‐water extraction procedure (LPS(Ph)) was added to PP cultures. When LPS prepared by a bntanol‐water extraction method was used, PP cultures from both C3H/HeN and C3H/HeJ mice were enhanced; however, the response of C3H/HeN PP cultures was significantly greater than that seen with C3H/HeJ PP cultures. The enhanced responsiveness of C3H/HeN PP cultures to MDP was probably due to an effect on the Mψ, since addition of MDP to either C3H/HeN or C3H/HeJ PP cultures containing C3H/HeN splenic adherent cells resulted in significantly enhanced immune responses. C3H/HeJ splenic Mψ did not promote adjuvant responses. LPS(Ph) augmentation of immune responses required that Mψ (spleen) and PP cultures be derived from LPS‐re‐sponsive C3H/HeN mice. These results demonstrated that Con A, MDP, and LPS promoted immune responses of PP cultures to the T‐dependent antigen, SRBC. Evidence is presented that Con A enhanced T‐helper‐cell activity, whereas MDP and LPS required the presence of LPS‐responsive Mψ for augmentation of immune responses. Copyright © 1982, Wiley Blackwell. All rights reserved
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  • Start Page

  • 329
  • End Page

  • 339
  • Volume

  • 15
  • Issue

  • 4