Kinetic analysis of cytokine-mediated receptor assembly using engineered FC heterodimers

Academic Article

Abstract

  • A method for analyzing ligand-receptor binding kinetics is described, which is based on an engineered FC domain (FChk) that forms a covalent heterodimer. To validate the system, the type I IFN receptors (IFNAR1 and IFNAR2) were expressed as IFNAR1-FChk, IFNAR2-FCkh, and IFNAR1/IFNAR2-FChk fusion proteins. Surface plasmon resonance (SPR) analysis of binary IFNa2a/ IFNAR interactions confirmed prior affinity measurements, while the affinity of the IFNa2a/IFNAR1/ IFNAR2-FChk interaction reproduced the affinity of IFNa2a binding to living cells. In cellular assays, IFNAR1/IFNAR2-FChk potently neutralized IFNa2a bioactivity with an inhibitory concentration equivalent to the KD measured by SPR. These studies suggest that FChk provides a simple reagent to evaluate the binding kinetics of multiple ligand-receptor signaling systems that control cell growth, development, and immunity. © 2013 The Protein Society.
  • Published In

  • Protein Science  Journal
  • Digital Object Identifier (doi)

    Author List

  • Deshpande A; Putcha BDK; Kuruganti S; Walter MR
  • Start Page

  • 1100
  • End Page

  • 1108
  • Volume

  • 22
  • Issue

  • 8