Subjection of ferritin subunits to reversed-phase HPLC effected a separation of them into three chromatographic species. These three putative subunit species were found in varying proportions from porcine spleen, liver, and heart ferritins as well as human liver, spleen, and placental ferritins. Detailed chemical-physical characterization of each of the isolated species indicate them to be two H-type and one L-type ferritin subunits. Thus, reversed-phase HPLC presently stands as the only available means to separate preparatively the various subunit types of ferritin. © 1986, Taylor & Francis Group, LLC. All rights reserved.