Overexpression and Purification of DNA Topoisomerase I from Yeast

Academic Article

Abstract

  • The mechanism of action of DNA topoisomerase I in catalyzing the relaxation of supercoiled DNA and how this reaction is perturbed by the antitumor drug camptothecin have been the subject of intense investigation (reviewedin (1-3). Much of this effort has focused on structure/function studies of wild-type and mutant forms of the enzyme, derived from a variety of sources (1,4-20). The budding yeast Saccharomyces cerevisiae has proven particularly amenable to the genetic manipulations required to overexpress and purify wild-type and mutant forms of eukaryotic DNA topoisomerase I (3,4,12,13).
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Bjornsti MA; Fertala J
  • Start Page

  • 179
  • End Page

  • 186
  • Volume

  • 94