Cellular strategies for regulating DNA supercoiling: A single-molecule perspective

Academic Article


  • Entangling and twisting of cellular DNA (i.e., supercoiling) are problems inherent to the helical structure of double-stranded DNA. Supercoiling affects transcription, DNA replication, and chromosomal segregation. Consequently the cell must fine-tune supercoiling to optimize these key processes. Here, we summarize how supercoiling is generated and review experimental and theoretical insights into supercoil relaxation. We distinguish between the passive dissipation of supercoils by diffusion and the active removal of supercoils by topoisomerase enzymes. We also review single-molecule studies that elucidate the timescales and mechanisms of supercoil removal. © 2010 Elsevier Inc.
  • Published In

  • Cell  Journal
  • Digital Object Identifier (doi)

    Author List

  • Koster DA; Crut A; Shuman S; Bjornsti MA; Dekker NH
  • Start Page

  • 519
  • End Page

  • 530
  • Volume

  • 142
  • Issue

  • 4