Splenic CD21high IGMhigh (MZ) B cells can quickly generate into plasma cells after in-vivo T-independent antigen stimulation

Academic Article


  • We have previously shown that CO21high IgMhigh mouse B cells enriched in the marginal zone (MZ) are unique in both phenotype and function compared to CD21int. IgMint. follicular (FO) B cells. In the VH81x transgenic mouse, a predominant heavy and light chain pair gives rise to a prevailing idiotype (Id) which is expressed in the majority of B cells in the MZ. These Id+ B cells function similarly to MZ B cells found in other normal mouse strains. Since Id+ B cells bind phosphorylcholine (PC), we investigated the response to a T-independent antigen from a heat killed Strep. pneumoniae vaccine in these transgenic mice. 24 hours following i.v. injection of the vaccine, syndecanhigh, cytoplasmic ╬╝high, CD38low, B220low plasma cells were observed in the red pulp and this response peaked at 3 days. These plasma cells were derived from MZ B cells as shown by expression of the predominant Id found in the VH81x MZ B cell population. Furthermore, the MZ B cells incorporated more BrdU than FO B cells 3 days after immunization, indicating that these cells were undergoing more rapid cellular division. Finally, serum Ig levels indicated that Id+ Ig was secreted following immunization. Thus MZ B cells are able to quickly respond to T-independent antigens by differentiating into plasma cells and secreting antigen specific Ig.
  • Author List

  • Oliver AM; Martin F; Kearney JF
  • Volume

  • 12
  • Issue

  • 5