Antibody-independent, CD4+ T-cell-dependent protection against pneumococcal colonization elicited by intranasal immunization with purified pneumococcal proteins

Academic Article


  • Immunity to pneumococcal colonization in mice by exposure to live or killed pneumococci has been shown to be antibody independent but dependent on CD4 + T cells. Here we show that intranasal immunization with pneumococcal proteins (pneumococcal surface protein C, adhesin A, and a pneumolysoid) can elicit a similar mechanism of protection. Colonization could be significantly reduced in mice congenitally deficient in immunoglobulins after intranasal immunization with this mixture of proteins; conversely, the depletion of CD4+ T cells in immunized wild-type mice at the time of challenge eliminated the protection afforded by immunization. Overall, our results show that intranasal immunization with a mixture of pneumococcal proteins protects against colonization in an antibody-independent, CD4 + T-cell-dependent manner. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 914905
  • Author List

  • Basset A; Thompson CM; Hollingshead SK; Briles DE; Ades EW; Lipsitch M; Malley R
  • Start Page

  • 5460
  • End Page

  • 5464
  • Volume

  • 75
  • Issue

  • 11