Quantitation of complement factor D in human serum by a solid-phase radioimmunoassay

Academic Article


  • A sensitive solid-phase radioimmunoassay is described which quantitates human D to 1-2 ng/ml. The assay was used to measure the concentration of D in normal and acute-phase sera and sera from individuals with systemic lupus erythematosus. All 3 groups of sera had comparable levels of D with mean values of 1.8, 2.3 and 2.5 μg/ml, respectively. Also tested were sera decomplemented in vitro by activators of the classical and alternative pathways. The results indicated that D is not depleted by alternative or classical pathway activation. However, heat inactivation (56°C, 30 min) of serum resulted in almost complete loss of antigenic D. © 1984.
  • Digital Object Identifier (doi)

    Pubmed Id

  • 3460411
  • Author List

  • Barnum SR; Niemann MA; Kearney JF; Volanakis JE
  • Start Page

  • 303
  • End Page

  • 309
  • Volume

  • 67
  • Issue

  • 2