Parvovirus Bl9-specific DNA in bone marrow from B19 arthropathy patients: Evidence for B19 virus persistence

Academic Article


  • To determine if B19 infection persists in patients with chronic B19 arthropathy, acute B19 infection in adults was documented by IgM serology. Bone marrow aspirates were obtained 24-42 months after acute infection in 4 patients who developed chronic joint symptoms. DNA from bone marrow aspirates was amplified by polymerase chain reaction by using B19-specific DNA sequences in the viral capsid gene and probing with B19-specific oligonucleotidesin Southern analysis. B19-specific DNA sequences were detected in all 4 chronic arthropathy patients compared with 0 of 7 anti-B19 IgM−, IgG− and 0 of 6 anti-B19 IgM−, IgG+ normal bone marrow donors. Three patients with serologically proven acute B19 infection and acute but nonchronic joint symptoms had B19 DNA detected in bone marrow aspirates 2-18 months after infection. These findings suggest that B19 arthropathy is associated with persistence of either B19 virus or select B19 DNA sequences. © 1993 by The University of Chicago.
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    Digital Object Identifier (doi)

    Author List

  • Foto F; Saag KG; Scharosch LL; Howard EJ; Naides SJ
  • Start Page

  • 744
  • End Page

  • 748
  • Volume

  • 167
  • Issue

  • 3