High-throughput flow cytometry screening reveals a role for junctional adhesion molecule a as a cancer stem cell maintenance factor

Academic Article

Abstract

  • Stem cells reside in niches that regulate the balance between self-renewal and differentiation. The identity of a stem cell is linked with the ability tointeract with its niche through adhesion mechanisms. To identify targets that disrupt cancer stemcell (CSC) adhesion, we performed a flow cytometryscreen on patient-derived glioblastoma (GBM) cells and identified junctional adhesion molecule A(JAM-A) as a CSC adhesion mechanism essentialfor self-renewal and tumor growth. JAM-A wasdispensable for normal neural stem/progenitorcell (NPC) function, and JAM-A expression was reduced in normal brain versus GBM. Targeting JAM-A compromised the self-renewal of CSCs. JAM-A expression negatively correlated to GBM patient prognosis. Our results demonstrate thatGBM-targeting strategies can be identified through screening adhesion receptors and JAM-A represents a mechanism for niche-driven CSC maintenance. © 2014 The Authors.
  • Published In

  • Cell Reports  Journal
  • Digital Object Identifier (doi)

    Author List

  • Lathia JD; Li M; Sinyuk M; Alvarado AG; Flavahan WA; Stoltz K; Rosager AM; Hale J; Hitomi M; Gallagher J
  • Start Page

  • 117
  • End Page

  • 129
  • Volume

  • 6
  • Issue

  • 1