Activation of glycogen phosphorylase and glycogenolysis in rat skeletal muscle by AICAR - An activator of AMP-activated protein kinase

Academic Article

Abstract

  • We determined whether the cell permeable molecule AICAR, whose metabolite activates AMP-activated protein kinase (AMPK) in cells, affected glycogen metabolism in rat soleus muscle preparations in vitro. The basal and insulin-stimulated rates of radiochemical lactate formation, net lactate release and glycogen synthesis were determined. AICAR stimulated net lactate release (but not radiochemical lactate formation) only at a basal concentration of insulin. An increased rate of glycogenolysis was the likely cause of increased net lactate release as glycogen phosphorylase activity vas significantly increased by AICAR. AICAR-stimulated net lactate release and phosphorylase activity were potently inhibited by insulin.
  • Authors

    Published In

  • FEBS Letters  Journal
  • Digital Object Identifier (doi)

    Author List

  • Young ME; Radda GK; Leighton B
  • Start Page

  • 43
  • End Page

  • 47
  • Volume

  • 382
  • Issue

  • 1-2