The induction of GSH synthesis by nanomolar concentrations of NO in endothelial cells: A role for γ-glutamylcysteine synthetase and γ-glutamyl transpeptidase

Academic Article


  • Nitric oxide protects cells from oxidative stress through a number of direct scavenging reactions with free radicals but the effects of nitric oxide on the regulation of antioxidant enzymes are only now emerging. Using bovine aortic endothelial cells as a model, we show that nitric oxide, at physiological rates of production (1-3 nM/s), is capable of inducing the synthesis of glutathione through a mechanism involving γ-glutamylcysteine synthetase and γ-glutamyl transpeptidase. This novel nitric oxide signalling pathway is cGMP-independent and we hypothesize that it makes an important contribution to the anti-atherosclerotic and antioxidant properties of nitric oxide. Copyright (C) 1999 Federation of European Biochemical Societies.
  • Published In

  • FEBS Letters  Journal
  • Digital Object Identifier (doi)

    Author List

  • Moellering D; Mc Andrew J; Patel RP; Forman HJ; Mulcahy RT; Jo H; Darley-Usmar VM
  • Start Page

  • 292
  • End Page

  • 296
  • Volume

  • 448
  • Issue

  • 2-3