Genetic recombination in the α2 domain of the Eα chain yields an Ed molecule with altered T cell activation

Academic Article


  • We have used a novel T cell selection strategy to isolate a mutant of an H‐2d/f murine macrophage line defective in its ability to present antigen to some Ed‐restricted helper T cells. This mutant has an amino acid substitution in the α2 domain of the Ed molecule. The mutation changes the sequence at codon 177 from ACC to CAC, which results in a threonine to histidine substitution and appears to be the first in vitro mutation to have arisen by genetic recombination. Even though the mutation is distal to the proposed antigen‐binding groove, it affects antigen presentation, presumably by altering the scaffolding for the antigen‐binding groove. This type of mutant might not be readily isolated using other selection techniques. Copyright © 1990 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Chang MY; Kowal C; Marzullo LR; Briner TJ; Gefter ML; Diamond B
  • Start Page

  • 2571
  • End Page

  • 2576
  • Volume

  • 20
  • Issue

  • 12