BH-protocadherin-c, a member of the cadherin superfamily, interacts with protein phosphatase 1 alpha through its intracellular domain

Academic Article

Abstract

  • Using a yeast two-hybrid system, we isolated eight cDNA clones which interacted with BH-protocadherin-c (BH-Pcdh-c) from the human brain cDNA library. One clone encoded protein phosphatase type 1 isoform α (PP1α) and another two PP1α2. PP1α was co-immunoprecipitated from the extract of a gastric adenocarcinoma cell line MKN-28 with anti-BH-Pcdh-c antibody. PP1α activity towards glycogen phosphorylase was inhibited by the intracellular domain of BH-Pcdh-c. Inhibition of the phosphatase required more than the minimal domain of BH-Pcdh-c which could associate with PP1α. In situ hybridization revealed that BH-Pcdh-c mRNA was predominantly expressed in cerebral cortex neurons in the adult mouse brain. Copyright (C) 1999 Federation of European Biochemical Societies.
  • Authors

    Published In

  • FEBS Letters  Journal
  • Digital Object Identifier (doi)

    Author List

  • Yoshida K; Watanabe M; Kato H; Dutta A; Sugano S
  • Start Page

  • 93
  • End Page

  • 98
  • Volume

  • 460
  • Issue

  • 1