A Novel Dual Lentiviral CRISPR-based Transcriptional Activation System for Gene Expression Regulation in Neurons.

Academic Article

Abstract

  • Robust and efficient gene expression control enables the study of a gene's function in the central nervous system. Advances in CRISPR-based technology provide new avenues not only for gene editing, but for complex transcriptional control. Here, we describe a protocol to generate high-titer lentiviruses with neuron-optimized CRISPR-activation constructs (dual lentiviruses consisting of a gene-specific single guide RNA and the CRISPR-activator) for use in primary neurons in vitro or in the adult brain in vivo. This protocol enables modular, scalable, and multiplexable gene regulation in the nervous system and does not require a transgenic model organism.
  • Authors

    Published In

  • Bio-protocol  Journal
  • Keywords

  • CRISPR activation, CRISPR-Cas9, Lentivirus, Neurons, Transcription
  • Digital Object Identifier (doi)

    Author List

  • Savell KE; Sultan FA; Day JJ
  • Start Page

  • e3348
  • Volume

  • 9
  • Issue

  • 17