Red cell interactions with amyloid-β1-40 fibrils in a murine model

Academic Article

Abstract

  • Vascular amyloidosis in Alzheimer's disease (AD) results in the exposure of red blood cells to β-amyloid fibrils (Aβ). The potential in vivo ramifications of this exposure have been investigated by injecting Aβ1-40 alone or Aβ-bound mouse red blood cells into the circulation of C57BL/6 mice. Results indicate that when Aβ1-40 is injected alone, a transient uptake of the fibrils by red blood cells occurs in vivo. When Aβ-bound red blood cells were injected, β-amyloid is rapidly removed from these cells in vivo. Double-labeling experiments indicate that while some of the red blood cells bound to Aβ1-40 are removed from circulation, a major fraction of these cells remain in circulation even after Aβ is removed. Immunohistochemistry of murine tissue samples obtained after sacrificing the animals suggests that within 1 h after injection of Aβ1-40 or Aβ-bound red blood cells, Aβ is found in spleen phagocytes and liver Kupffer cells. Heme staining further indicates that the binding of Aβ1-40 to red blood cells enhances red cell phagocytosis by the spleen. © 2004 Elsevier Inc. All rights reserved.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Ravi LB; Poosala S; Ahn D; Chrest FJ; Spangler EL; Jayakumar R; Nagababu E; Mohanty JG; Talan M; Ingram DK
  • Start Page

  • 28
  • End Page

  • 37
  • Volume

  • 19
  • Issue

  • 1-2