Comparative Gene Expression Analyses Reveal Distinct Molecular Signatures between Differentially Reprogrammed Cardiomyocytes

Academic Article


  • Cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) or directly reprogrammed from non-myocytes (induced cardiomyocytes [iCMs]) are promising sources for heart regeneration or disease modeling. However, the similarities and differences between iPSC-CMs and iCMs are still unknown. Here, we performed transcriptome analyses of beating iPSC-CMs and iCMs generated from cardiac fibroblasts (CFs) of the same origin. Although both iPSC-CMs and iCMs establish CM-like molecular features globally, iPSC-CMs exhibit a relatively hyperdynamic epigenetic status, whereas iCMs exhibit a maturation status that more closely resembles that of adult CMs. Based on gene expression of metabolic enzymes, iPSC-CMs primarily employ glycolysis, whereas iCMs utilize fatty acid oxidation as the main pathway. Importantly, iPSC-CMs and iCMs exhibit different cell-cycle status, alteration of which influenced their maturation. Therefore, our study provides a foundation for understanding the pros and cons of different reprogramming approaches. Zhou et al. performed gene expression profiling of cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) or generated through direct reprogramming (induced cardiomyocytes [iCMs]) from cardiac fibroblasts of the same origin. Comparative analyses revealed similarities and differences in the molecular signatures of iPSC-CMs and iCMs.
  • Published In

  • Cell Reports  Journal
  • Digital Object Identifier (doi)

    Author List

  • Zhou Y; Wang L; Liu Z; Alimohamadi S; Yin C; Liu J; Qian L
  • Start Page

  • 3014
  • End Page

  • 3024
  • Volume

  • 20
  • Issue

  • 13