Human interleukin-12 enhances interferon-γ-producing influenza-specific memory CD8+ cytotoxic T lymphocytes

Academic Article


  • Interferon (IFN)-γ synthesis of CD45RO+ (memory) and CD45RA+ (naive) CD8+ cytotoxic T lymphocytes (CTLs) and the role of interleukin (IL)-12 in the regulation of human CTL functions in virus-specific immunity were investigated. After culture with influenza virus, CD45RO+ CD8+ T cells from human peripheral blood mononuclear cells increased in frequency and exhibited significant major histocompatibility complex class I-mediated CTL activity, whereas CD45RA+ CD8+ T cells did not. Influenza virus-stimulated CD45RO+ CD8+ T cells contained significantly higher levels of IFN-γ-producing cells and IFN-γ-specific mRNA than did CD45RA+ CD8+ T cells. Recombinant human IL-12 further enhanced CTL activity and IFN-γ production by CD45RO+ CD8+ T cells. These data clearly show that human virus-specific CTL activity and coproduction of IFN-γ are associated with the CD45RO+ CD8+ T cells that are modulated by the cell-mediated, immunity-inducible cytokine IL-12 in humans.
  • Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 21458447
  • Author List

  • Mbawuike IN; Fujihashi K; DiFabio S; Kawabata S; McGhee JR; Couch RB; Kiyono H
  • Start Page

  • 1477
  • End Page

  • 1486
  • Volume

  • 180
  • Issue

  • 5