A simple and reliable method for determination of optimum pH in coupled enzyme assays

Academic Article


  • Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes. Here, we demonstrate a simple and reliable method to determine the pH optimum for pyruvate kinase using the pyruvate kinase/lactate dehydrogenase coupled enzyme assay. This simple and reliable method can be broadly adapted to determine the pH optimum for various enzymes that are assayed using a coupled enzyme assay.
  • Digital Object Identifier (doi)

    Author List

  • Bowman L; Motamed R; Lee P; Aleem K; Berawala AS; Hayden KL; Bzik DJ; Chattopadhyay D
  • Start Page

  • 200
  • End Page

  • 203
  • Volume

  • 68
  • Issue

  • 4