A single column method to measure hydrogen--tritium exchange by gel chromatography.

Academic Article


  • Employment of a commercially integrated gel chromatography system together with the utilization of cross-linked polyacrylamide as the chromatographic medium simplifies the methodology of hydrogen--tritium exchange measurements. The system described allows the execution of hydrogen--tritium exchange measurements with as little as 0.5 mg protein per time point and with only a single pass of sample through the column for out-exchange times of less than 1 min to at least 24 h. The accuracy and precision of this system are comparable to those of existing methodologies.
  • Published In


  • Chemical Phenomena, Chemistry, Physical, Chromatography, Gel, Ferritins, Hydrogen, Tritium
  • Digital Object Identifier (doi)

    Pubmed Id

  • 2200804
  • Author List

  • Collawn JF; Fish WW
  • Start Page

  • 311
  • End Page

  • 315
  • Volume

  • 7
  • Issue

  • 4