Methylation-specific polymerase chain reaction (PCR) for gene-specific DNA methylation detection

Academic Article


  • Methylation-specific polymerase chain reaction (MS-PCR) is a more rapid way to detect changes in DNA methylation than is bisulfite sequencing. In addition, by incorporating some basic automation, samples can be prepared and analyzed in a 96-well plate format. The method can be used either quantitatively (qRT-PCR-based MethyLight) or qualitatively (using agarose gels) to detect changes in DNA methylation; both are described in this protocol.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Lizardi PM; Yan Q; Wajapeyee N
  • Start Page

  • 1019
  • End Page

  • 1022
  • Volume

  • 2017
  • Issue

  • 12