Expression of c-myc, c-myb, and c-sis in fibroblasts from affected and unaffected skin of patients with systemic sclerosis

Academic Article

Abstract

  • We examined c-sis, c-myc, and c-myb proto-oncogene expression in fibroblasts cultured from affected and unaffected skin of patients with systemic sclerosis (SSc), and from healthy donor skin. Total cellular RNA from cultured dermal fibroblasts was used in slot blot analysis and scanning densitometry or phosphorimaging to quantify steady-state levels of proto-oncogene mRNAs. PDGF B-chain levels in culture supematants of fibroblasts were determined by ELISA. Our results demonstrate that steady-state levels of c-myc and c-myb mRNA were elevated 1.5-to 5.6-fold in intralesional fibroblasts from SSc patients as compared to other cells examined. Levels of c-sis mRNA and PDGF-B protein were comparable regardless of source. Elevated c-myc and c-myb expression may be indicative of, and may contribute to, fibroblast activation in SSc. © 1993 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
  • Authors

    Published In

  • Autoimmunity  Journal
  • Digital Object Identifier (doi)

    Author List

  • Feghali CA; Boulware DW; Ferriss JA; Levy LS
  • Start Page

  • 167
  • End Page

  • 171
  • Volume

  • 16
  • Issue

  • 3