Main structures of the forssman glycolipid hapten and a leb-like glycolipid of dog small intestine, as revealed by mass spectrometry. Difference in ceramide structure related to tissue localization

Academic Article

Abstract

  • Two glycolipids of dog small intestine, one with Forssman activity and one with Leb-like activity, have been characterized by mass spectrometry of methylated, and methylated and reduced (LiAlH4) derivatives. The Forssman glycolipid was conclusively shown to be a pentaglycosylceramide with the carbohydrate sequence hexosamine-hexosamine-hexose-hexose-hexose-ceramide, and with sphingosine (dihydroxy base) as major long-chain base and normal fatty acids as the only fatty acids. The Leb -like glycolipid was a hexaglycosylceramide with the sequence fucose-hexose-[fucose-] hexosamine-hexosehexose -ceramide and with phytosphingosine (trihydroxy base) as major longchain base and only 2-hydroxy fatty acids as fatty acids. The difference of two hydroxy groups in the ceramide between the two glycolipids may be related to a different tissue localization. As shown by immunofluorescens studies the Forssman activity was associated with the lamina propria and the Leb-like activity to the glandular epithelium of dog small intestine. © 1975.
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    Author List

  • Smith EL; Mckibbin JM; Karlsson KA; Pascher I; Samuelsson BE
  • Start Page

  • 171
  • End Page

  • 179
  • Volume

  • 388
  • Issue

  • 2