Solid Medium For the Genetic Manipulation of Thiobacillus Ferrooxidans

Academic Article


  • A new solid medium, termed solid 2:2 medium, was developed for application to the genetic manipulation of Thiobacillus ferrooxidans. The medium contains a mixture of both ferrous iron and thiosulphate as energy sources for the growth of T. ferrooxidans at pH 4.6-4.8. Kanamycin and streptomycin could be used in the medium to select recombinants. Thousands of interspersing colonies can develop on each plate without any iron-oxidizing zones. T. ferrooxidans colonies on solid 2: 2 medium have distinct morphologies, and several types of colonies were described. A new type of colony morphology variant with high mutation and reversion rates was also observed. © 1994, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation. All rights reserved.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Peng JB; Yan WM; Bao XZ
  • Start Page

  • 243
  • End Page

  • 253
  • Volume

  • 40
  • Issue

  • 3