DNA binding proteins of the filamentous phages CTXphi and VGJphi of Vibrio cholerae.

Academic Article

Abstract

  • The native product of open reading frame 112 (orf112) and a recombinant variant of the RstB protein, encoded by Vibrio cholerae pathogen-specific bacteriophages VGJphi and CTXphi, respectively, were purified to more than 90% homogeneity. Orf112 protein was shown to specifically bind single-stranded genomic DNA of VGJphi; however, RstB protein unexpectedly bound double-stranded DNA in addition to the single-stranded genomic DNA. The DNA binding properties of these proteins may explain their requirement for the rolling circle replication of the respective phages and RstB's requirement for single-stranded-DNA chromosomal integration of CTXphi phage dependent on XerCD recombinases.

    • Authors

    Published In

    Keywords

  • Attachment Sites, Microbiological, Bacteriophages, DNA, DNA, Single-Stranded, DNA-Binding Proteins, Electrophoresis, Polyacrylamide Gel, Gene Expression Regulation, Viral, Inovirus, Open Reading Frames, Recombinant Proteins, Vibrio cholerae, Viral Proteins, Virus Integration

    • Digital Object Identifier (doi)

    Author List

  • Falero A; Caballero A; Ferrán B; Izquierdo Y; Fando R; Campos J

    • Start Page

  • 5873

    • End Page

  • 5876

    • Volume

  • 191

    • Issue

  • 18