FoxO1 represses LXRα-mediated transcriptional activity of SREBP-1c promoter in HepG2 cells

Academic Article


  • Recent studies have demonstrated that FoxO1 modulates the expression of SREBP-1c, but the exact mechanism remains unknown. Our results demonstrate that FoxO1 suppresses the SREBP-1c promoter transcriptional activity in HepG2 cells. This repression was independent of FoxO1 binding to the SREBP-1c promoter, but LXR responsive elements (LXREs) were crucial to this phenomenon. Moreover, FoxO1 also strongly inhibited the LXRα-mediated elevated transcription by SREBP-1c promoter. Electrophoretic mobility shift assay and chromatin immuno-precipitation further suggested the ability of FoxO1 to inhibit LXRα binding with the LXRE in the SREBP-c promoter. FoxO1-mediated suppression of SREBP-1c promoter activity could be partially alleviated by insulin. © 2010 Federation of European Biochemical Societies.
  • Authors

    Published In

  • FEBS Letters  Journal
  • Digital Object Identifier (doi)

    Pubmed Id

  • 25315148
  • Author List

  • Liu X; Qiao A; Ke Y; Kong X; Liang J; Wang R; Ouyang X; Zuo J; Chang Y; Fang F
  • Start Page

  • 4330
  • End Page

  • 4334
  • Volume

  • 584
  • Issue

  • 20