Technical note: Discordant analytical results caused by biotin interference on Diagnostic immunoassays in a pediatric hospital

Academic Article


  • Recent studies have reported that biotin interferes with certain immunoassays. In this study, we evaluated the analytical interference of biotin on immunoassays that use streptavidin-biotin in our pediatric hospital. We tested the effect of different concentrations of biotin (1.5-200 ng/ml) on TSH, Prolactin, Ferritin, CK-MB, β-hCG, Troponin I, LH, FSH, Cortisol, Anti-HAV antibody (IgG and IgM), assays on Ortho Clinical Diagnostic Vitros 5600 Analyzer. Biotin (up to 200 ng/mL) did not significantly affect Troponin I and HAV assays. Biotin (up to 12.5 ng/ml) resulted in < 10% bias in CK-MB, β-hCG, AFP, Cortisol, Ferritin assays and biotin > 6.25 ng/mL significantly affected TSH (> 20% bias) assay. Prolactin was significantly affected even at low levels (Biotin 1.5 ng/mL).Thus, we recommend educating physicians about biotin interference in common immunoassays and adding an electronic disclaimer.
  • Authors

    Author List

  • Ali M; Rajapakshe D; Cao L; Devaraj S
  • Start Page

  • 638
  • End Page

  • 640
  • Volume

  • 47
  • Issue

  • 5