Bridging the gap: Super-resolution microscopy of epithelial cell junctions

Academic Article


  • Cell junctions are critical for cell adhesion and communication in epithelial tissues. It is evident that the cellular distribution, size, and architecture of cell junctions play a vital role in regulating function. These details of junction architecture have been challenging to elucidate in part due to the complexity and size of cell junctions. A major challenge in understanding these features is attaining high resolution spatial information with molecular specificity. Fluorescence microscopy allows localization of specific proteins to junctions, but with a resolution on the same scale as junction size, rendering internal protein organization unobtainable. Super-resolution microscopy provides a bridge between fluorescence microscopy and nanoscale approaches, utilizing fluorescent tags to reveal protein organization below the resolution limit. Here we provide a brief introduction to super-resolution microscopy and discuss novel findings into the organization, structure and function of epithelial cell junctions.
  • Published In

  • Tissue Barriers  Journal
  • Digital Object Identifier (doi)

    Author List

  • Bartle EI; Rao TC; Urner TM; Mattheyses AL
  • Volume

  • 6
  • Issue

  • 1