Crystallization and preliminary X-ray investigation of uridine phosphorylase from Escherichia coli.

Academic Article


  • Crystals of uridine phosphorylase from Escherichia coli K12 have been grown from solutions of polyethylene glycol 4000. The crystals are trigonal, space group R3; the hexagonal axes are a = 154.4 A and c = 49.4 A. The crystals are quite stable to x-rays and diffract beyond 2.6 A resolution. It appears that the molecule is a hexamer with a subunit molecular weight of 27,500 and utilizes the 3-fold symmetry of the space group, resulting in two subunits/asymmetric unit.
  • Published In

    Author List

  • Cook WJ; Koszalka GW; Hall WW; Narayana SV; Ealick SE
  • Start Page

  • 2852
  • End Page

  • 2853
  • Volume

  • 262
  • Issue

  • 6