Application of the polymerase chain reaction to the diagnosis and management of neonatal herpes simplex virus disease

Academic Article


  • Cerebrospinal fluid (CSF) specimens from 77 neonates with herpes simplex virus (HSV) disease were evaluated retrospectively by polymerase chain reaction (PCR). Samples were collected from 202 infants enrolled in a National Institute of Allergy and Infectious Diseases Collaborative Antiviral Study Group trial that compared vidarabine with acyclovir for the treatment of neonatal HSV infection. HSV DNA was detected in the CSF of 26 (76%) of 34 infants with CNS disease, in 13 (93%) of 14 infants with disseminated infection, and in 7 (24%) of 29 with skin, eye, or mouth (SEM) involvement. One of the 7 PCR-positive SEM patients subsequently developed severe neurologic impairment. Eighteen (95%) of 19 infants with positive CSF PCR results after the completion of 10 days of antiviral therapy experienced significant morbidity or mortality. Application of PCR to neonatal HSV disease may provide additional information on which clinical decisions may be based, although its diagnostic utility outside the research setting is unproven.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Kimberlin DW; Lakeman FD; Arvin AM; Prober CG; Corey L; Powell DA; Burchett SK; Jacobs RF; Starr SE; Whitley RJ
  • Start Page

  • 1162
  • End Page

  • 1167
  • Volume

  • 174
  • Issue

  • 6