Depletion of substance P from rat primary sensory neurons by ATP, an implication of P2X receptor-mediated release of substance P

Academic Article


  • Effects of ATP on substance P immunoreactivity were examined in cultured dorsal root ganglion neurons. We found that treatment of dorsal root ganglion neurons with ATP significantly depleted substance P immunoreactivity on the neurites and somata of the neurons. The effects of ATP were significantly inhibited by the purinergic P2 receptor antagonists suramin (30 μM) and pyridoxal-phosphate-6-azophenyl-2′,4′-disulfonic acid (10 μM). We also showed that ATP-induced depletion of substance P immunoreactivity from dorsal root ganglion neurons depended on the entry of Ca2+. In a spinal cord slice preparation, we also found the internalization of neurokinin-1/substance P receptors in many dorsal horn neurons following the application of ATP or α,β-methylene-ATP. Together these results indicate that activation of P2X receptors may result in release of substance P from primary afferent neurons. © 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.
  • Authors

    Published In

  • Neuroscience  Journal
  • Digital Object Identifier (doi)

    Author List

  • Nakatsuka T; Mena N; Ling J; Gu JG
  • Start Page

  • 293
  • End Page

  • 300
  • Volume

  • 107
  • Issue

  • 2