Breast milk human cytomegalovirus (CMV) viral load and the establishment of breast milk CMV-pp65-Specific CD8 T Cells in Human CMV infected mothers

Academic Article


  • The role of human cytomegalovirus (HCMV)-specific T-cell responses in breast milk of HCMV-seropositive mothers is not well defined. In these studies, we demonstrate that the frequency of cytomegalovirus (CMV)-pp65-specific T-cell responses in peripheral blood mononuclear cells (PBMCs) and breast milk cells (BMCs) is increased for CD8+ T cells in both sample sources when compared with CD4+ T cells. The frequency of pp55-specific CD8 T cells producing interferon γ (IFN-γ) alone or dual IFN-γ/granzyme rB producers is increased in breast milk compared with PBMCs. Lastly, we observed a positive correlation between breast milk viral load and the CD8 pp65-specific response, suggesting that local virus replication drives antigen-specific CD8 T cells into the breast.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Moylan DC; Pati SK; Ross SA; Fowler KB; Boppana SB; Sabbaj S
  • Start Page

  • 1176
  • End Page

  • 1179
  • Volume

  • 216
  • Issue

  • 9