The Effect of 1-β-D-Arabinofuranosylcytosine on Cell Viability, DNA Synthesis, and Chromatid Breakage in Synchronized Hamster Fibrosarcoma Cells

Academic Article

Abstract

  • Hamster fibrosarcoma cells were synchronized by mitotic selection and exposed to varying concentrations of 1-β-d-arabinofuranosylcytosine (ara-C) for 2 hr in mid-S phase. There was a direct relationship between DNA synthesis inhibition and cytotoxicity produced by ara-C once DNA synthesis was decreased by over 85%. The noncytotoxic concentration of 10 -5 m ara-C produced little chromatid breakage; but extensive chromatid breakage and chromosomal rearrangement were seen in cells treated with the cytotoxic concentration of 10 -3 m ara-C, thus supporting earlier observations that chromatid breakage is highly correlated with cytotoxicity. Predominantly small DNA was synthesized when cells were treated with both 10 -5 and 10 - 3 m ara-C, and this DNA could be completely chased into high-molecular-weight DNA after addition of deoxycytidine. Both concentrations of ara-C also inhibited, to different degrees, the joining of intermediate-size DNA fragments into larger DNA; thus neither parameter appeared directly related to the ara-C-produced cytotoxicity. © 1976, American Association for Cancer Research. All rights reserved.
  • Authors

    Published In

  • Cancer Research  Journal
  • Author List

  • Jones PA; Benedict WF
  • Start Page

  • 3789
  • End Page

  • 3797
  • Volume

  • 36
  • Issue

  • 10