The effect of plasma solutes on total-body-water measurements via NMR.

Academic Article


  • Nuclear magnetic resonance (NMR) spectroscopy has been reported as an alternative method for quantitating deuterium oxide concentrations in the evaluation of total-body-water in humans. However, the presence of dissolved plasma proteins results in an underestimation of deuterium NMR (2H-NMR) intensity ratios, thereby causing an overestimation (5-6%) of total-body-water (TBW) values determined from nonsublimed patient plasma samples. We demonstrate that plasma samples must be corrected for the volume percentage of water in plasma. Correction of initial 2H-NMR intensity ratios with a factor of 0.93 results in intensity ratios comparable to those determined from plasma samples subjected to vacuum sublimation to remove all plasma solutes.
  • Authors

    Published In

  • Nutrition  Journal
  • Author List

  • Richards EW; Abu Khaled M; Watkins CL; Long CL
  • Start Page

  • 344
  • End Page

  • 346
  • Volume

  • 7
  • Issue

  • 5