Evaluation of two ELISA's for detecting Chlamydia trachomatis from endocervical swabs

Academic Article

Abstract

  • Two enzyme immunoassays (EIAs) detecting Chlamydia trachomatis from endocervical swabs, Syva MicroTrak (MT) and Abbott Chlamydiazyme (CZ), were compared with a tissue culture (TC) standard. Initially, 8% (100 of 1250) of specimens were TC positive, yielding sensitivities of 94% (94 of 100) for MT and 79% (79 of 100) for CZ with identical 98% specificities (1129 of 1150 for MT and 1130 of 1150 for CZ). Discrepant specimens were retested by both EIAs and assayed for elementary bodies (EBs) by a fluorescent antibody test. After discrepancy analysis, 9.5% (118) of 1240 patients were either TC or EB positive, yielding sensitivities of 94.1% for MT (111 of 118) and 79.7% for CZ (94 of 118) with identical specificities of 100% (1122 of 1122). These results indicate that the MT is significantly more sensitive (p < 0.05, McNemar test) than CZ in detecting C. trachomatis from endocervical swabs. © 1992.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Altaie SS; Meier FA; Centor RM; Wakabongo M; Toksoz D; Harvey KM; Basinger E; Johnson BA; Brookman RR; Dalton HP
  • Start Page

  • 579
  • End Page

  • 586
  • Volume

  • 15
  • Issue

  • 7