Methods of assessing factor VIII content of stored fresh frozen plasma intended for preparation of factor VIII concentrates.

Academic Article


  • Minimally destructive methods were sought to assess the factor VIII content of fresh frozen plasma intended for large-scale fractionation and stored in five-liter polyethylene packs after pooling approximately 23 plasma donations. Although factor VII distribution in the frozen pack was not perfectly homogenous, a central "core" through the frozen pack gave a representative sampel of the entire contents of the pack. Coring was compared with other methods of pack sampling before large-scale cryoprecipitation. The survival of factor VIII was studied in three grades of stored plasma which had been separated and frozen within 4 hours, 8 hours, and 18 hours of blood donation. Plasma frozen after overnight storage of blood is a satisfacory starting material for the preparation of factor VIII and factor IX concentrates.
  • Published In

  • Transfusion  Journal
  • Keywords

  • Blood Banks, Blood Preservation, Factor VIII, Freezing, Humans
  • Digital Object Identifier (doi)

    Author List

  • Smith JK; Snape TJ; Haddon ME; Gunson HH; Edwards R
  • Start Page

  • 530
  • End Page

  • 537
  • Volume

  • 18
  • Issue

  • 5