Direct profiling of environmental microbial populations by thermal dissociation analysis of native rRNAs hybridized to oligonucleotide microarrays

Academic Article

Abstract

  • Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • El Fantroussi S; Urakawa H; Bernhard AE; Kelly JJ; Noble PA; Smidt H; Yershov GM; Stahl DA
  • Start Page

  • 2377
  • End Page

  • 2382
  • Volume

  • 69
  • Issue

  • 4