Nanoarray-surfaces by reconstitution of the porin MspA into stabilized long-chain-lipid-monolayers at a gold-surface

Academic Article

Abstract

  • We have reconstituted the channel porin MspA from M. smegmatis on a gold electrode into a long-chain lipid monolayer resembling a biological membrane. The tailored deposition of the self-assembled monolayer (SAM) on gold was achieved by employing electrochemically initiated generation of thiyl-radicals from organic thiosulfates (Bunte-Salts), which then adsorb onto the gold's surface. At surface coverage of the lipids of approximately 80%, MspA reconstituted efficiently from the aqueous buffer into the SAM. The combination of cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), reflection electron microscopy (REM) and the formation of copper(0)- nanoparticles from copper(II)-ions inside the MspA-channels proved that MspA forms SAM-spanning ion channels on gold, keeping the electrode (surface) accessible through the pores. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
  • Published In

  • Electroanalysis  Journal
  • Digital Object Identifier (doi)

    Author List

  • Wörner M; Niebler S; Gogritchiani E; Egner N; Braun AM; Niederweis M; Bossmann SH
  • Start Page

  • 1859
  • End Page

  • 1870
  • Volume

  • 18
  • Issue

  • 19-20