Separation of Cells with Histochemically Demonstrable Acid Phosphatase Activity from Suspensions of Cells from Human Prostatic Carcinomas in an lsokinetic Gradient of Ficoll in Tissue Culture Medium

Academic Article

Abstract

  • This report describes the separation of cells exhibiting histochemically demonstrable acid phosphatase from suspensions of cells obtained from human prostatic carcinomas by velocity sedimentation. In the unseparated suspensions of cells, 40.5 ± 7.7% of nucleated cells contained histochemically evident acid phosphatase. After cells were separated by velocity sedimentation, 86.4 ± 9.4% of the nucleated cells in the purest fractions exhibited histochemically demonstrable acid phosphatase activity. More than 95% of these cells excluded trypan blue. To our knowledge, this is the first report of a method for the separation of viable epithelial cells from human prostatic carcinomas. © 1976, American Association for Cancer Research. All rights reserved.
  • Authors

    Published In

  • Cancer Research  Journal
  • Pubmed Id

  • 10933493
  • Author List

  • Helms SR; Pretlow TG; Bueschen AJ; Lloyd KL; Murad TM
  • Start Page

  • 481
  • End Page

  • 486
  • Volume

  • 36