Culture of normal human anterior pituitary cell monolayers

Academic Article


  • A detailed procedure is described for the culture of functional normal human anterior pituitary cell monolayers for periods up to 2 months. The tissue is dispersed using a double digestion method using collagenase and hyaluronidase followed by trypsin. Attachment of the cells is accomplished in 5 to 9 d. Verification of the presence of functional cells is demonstrated by release of prolactin and luteinizing hormone in response to appropriate stimuli. © 1983 Tissue Culture Association, Inc.
  • Digital Object Identifier (doi)

    Pubmed Id

  • 18955731
  • Author List

  • Rodgers-Neame NT; Blackwell RE
  • Start Page

  • 181
  • End Page

  • 186
  • Volume

  • 7
  • Issue

  • 4