Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells

Academic Article

Abstract

  • We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation profiles and differentiation potential. Extended passage of some iPSC clones in culture did not improve their epigenetic resemblance to embryonic stem cells, implying that some human iPSCs retain a residual 'epigenetic memory' of their tissue of origin. © 2011 Nature America, Inc. All rights reserved.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 10896871
  • Author List

  • Kim K; Zhao R; Doi A; Ng K; Unternaehrer J; Cahan P; Hongguang H; Loh YH; Aryee MJ; Lensch MW
  • Start Page

  • 1117
  • End Page

  • 1119
  • Volume

  • 29
  • Issue

  • 12