Transformation of Mycoplasma capricolum and examination of DNA restriction modification in M. capricolum and Mycoplasma mycoides subsp. mycoides

Academic Article


  • Plasmids pIKΔ and pIKΔ-erm have recently been developed as mycoplasmal cloning vectors. In this report, we demonstrate that these plasmids can replicate in Mycoplasma capricolum, a mycoplasmal species for which transformation had not previously been characterized. Both plasmids are stably maintained at a higher copy number than in their parental species. Mycoplasma mycoides subsp. mycoides. We have also examined the possibility of one or more restriction-modification systems affecting transformation frequencies in both species. © 1994 Academic Press, Inc.
  • Published In

  • Plasmid  Journal
  • Digital Object Identifier (doi)

    Author List

  • King KW; Dybvig K
  • Start Page

  • 308
  • End Page

  • 311
  • Volume

  • 31
  • Issue

  • 3