Nucleotide sequence of Mycoplasma mycoides subspecies Mycoides plasmid pKMK1

Academic Article


  • To facilitate the development of mycoplasmal cloning vectors, we have determined the nucleotide sequence of pKMK1, a cryptic plasmid isolated from Mycoplasma mycoides subsp. mycoides. It is 1875 bp in length and contains two open reading frames (ORFs) that share homology with ORFs from members of a large family of gram-positive bacterial plasmids which replicate via a single-stranded DNA intermediate. Putative origins of replication and candidate cloning sites have been identified. © 1992.
  • Published In

  • Plasmid  Journal
  • Digital Object Identifier (doi)

    Author List

  • King KW; Dybvig K
  • Start Page

  • 86
  • End Page

  • 91
  • Volume

  • 28
  • Issue

  • 1