A chicken repetitive DNA sequence that is highly sensitive to single-strand specific endonucleases

Academic Article


  • A DNA sequence consisting of the 5-mer AGAGG repeated tandemly 32 times has been detected in a chicken genome clone and found to be present in about 2000 copies per chicken genome. This sequence was highly susceptible to single-strand specific endonucleases isolated from Aspergillus oryzae (S1) and mung bean, but cleavage by a single-strand specific endonuclease isolated from Neurospora crassa occurred only at a pH below 5.5. Endonucleolytic cutting of the AGAGG sequence by the single-strand specific enzymes required a supercoiled substrate and was independent of ionic strength. © 1983 IRL Press Limited.
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    Digital Object Identifier (doi)

    Author List

  • Dybvig K; Clark CD; Aliperti G; Schlesinger MJ
  • Start Page

  • 8495
  • End Page

  • 8508
  • Volume

  • 11
  • Issue

  • 23