Rapid screening and identification of cytochalasins by electrospray tandem mass spectrometry

Academic Article


  • The cytochalasin class of fungal metabolites was analyzed by electrospray ionization tandem mass spectrometry (ESI-MS/MS) with the aim of developing a methodology for their rapid identification in microbial extracts. ESI-MS analyses of reference cytochalasins were performed and several product ions were produced in MS/MS experiments on parent ions that are structurally characteristics. A precursor ion search was performed to detect cytochalasins in an ethyl acetate extract of fungal strain RK97-F21. Three cytochalasins were detected and one of the components was identified as epoxycytochalasin H by comparing the tandem mass spectra of the product ions with those of reference compounds. This finding was further validated by LC/MS and LC/MS/MS experiments. Copyright © 2002 John Wiley & Sons, Ltd.
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    Digital Object Identifier (doi)

    Author List

  • Prasain JK; Ueki M; Stefanowicz P; Osada H
  • Start Page

  • 283
  • End Page

  • 291
  • Volume

  • 37
  • Issue

  • 3