VIP-expressing dendritic cells protect against spontaneous autoimmune peripheral polyneuropathy.

Academic Article


  • The spontaneous autoimmune peripheral polyneuropathy (SAPP) model in B7-2 knockout nonobese diabetic mice mimics a progressive and unremitting course of chronic inflammatory demyelinating polyradiculoneuropathy (CIDP). In this study, bone marrow-derived dendritic cells (DCs) were transduced to express vasoactive intestinal polypeptide (VIP) using a lentiviral vector (LV-VIP). These transduced DCs (LV-VIP-DCs) were then injected intravenously (i.v.) into 16-week-old (before disease onset) and 21-week-old (after disease onset) SAPP mice in order to prevent or attenuate the disease. Outcome measures included behavioral tests, clinical and histological scoring, electrophysiology, real-time PCR, flow cytometry analyses, and enzyme-linked immunosorbent assay. LV-VIP-DCs were recruited to the inflamed sciatic nerve and reduced the expression of inflammatory cytokines. A single injection of LV-VIP-DC delayed the onset of disease, stabilized, and attenuated clinical signs correlating with ameliorated behavioral functions, reduced nerve demyelination, and improved nerve conduction. This proof-of-principle study is an important step potentially leading to a clinical translational study using DCs expressing VIP in cases of CIDP refractory to standard immunosuppressive therapy.
  • Published In

  • Molecular Therapy  Journal
  • Keywords

  • Animals, Cells, Cultured, Dendritic Cells, Male, Mice, Peripheral Nervous System Diseases, Polyneuropathies, Vasoactive Intestinal Peptide
  • Digital Object Identifier (doi)

    Author List

  • Yalvac ME; Arnold WD; Hussain S-RA; Braganza C; Shontz KM; Clark KR; Walker CM; Ubogu EE; Mendell JR; Sahenk Z
  • Start Page

  • 1353
  • End Page

  • 1363
  • Volume

  • 22
  • Issue

  • 7